Special stains in study of infertile endometrium

Authors

  • Mohasin Jakirhusain Halgale Department of Pathology, R.C.S.M. Government Medical College, Kolhapur, India
  • Meenal Vitthal Jadhav Department of Pathology, B.J. Medical College, Pune, India
  • Nalini V Kadgi Department of Pathology, B.J. Medical College, Pune, India

Keywords:

Endometrium, Infertility, Special stains

Abstract

Background: Integrity of ovarian function is a prerequisite for successful implantation of ovum. The subtle changes in ovarian function can be detected by studying endometrial carbohydrates with special stains.

Material and methods: 87 infertile and 30 normal endometria were studied with the help of Hematoxylin& Eosin ( H & E) stain and special stains like Best Carmine, Alcian blue PAS (with and without diastase digestion) and reticulin stain.

Results: Special stains were useful in emphasizing the cyclical activity of carbohydrates and stroma in normal endometrium. In infertile endometrium, they helped in precise classification and detection of corpus luteal deficiency, at times (8.7% cases) missed on routine H& E stain . Intrinsic abnormalities like polyps and tuberculous granulomas could be detected with ease on reticulin stain. On application of special stains, incidence of corpus luteal deficiency was 27.5%, and that of anovulatory infertility and normal secretory endometrium was 34.5% each. 

Conclusion: The special stains helped in detecting precise endometrial response and added to the information obtained by routine H&E stain.

DOI:10.21276/APALM.1725

References

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Published

28-04-2018

How to Cite

1.
Halgale MJ, Jadhav MV, Kadgi NV. Special stains in study of infertile endometrium. Ann of Pathol and Lab Med [Internet]. 2018 Apr. 28 [cited 2024 Dec. 27];5(4):A301-304. Available from: https://pacificejournals.com/journal/index.php/apalm/article/view/1725

Issue

Vol. 5 No. 4 (2018)

Section

Original Article

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Copyright (c) 2018 Mohasin Jakirhusain Halgale, Meenal Vitthal Jadhav, Nalini V Kadgi

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