Cytospin preparation from residual material in needle hub: Does it add to fine needle aspiration diagnosis?

Authors

  • Shilpa Gupta Hindu Rao Hospital
  • Lopamudra Deka Hindu Rao Hospital
  • Ruchika Gupta Chacha Nehru Bal Chikitsalaya
  • Kusum Gupta Hindu Rao Hospital
  • Charanjeet Kaur Hindu Rao Hospital
  • Sompal Singh Hindu Rao Hospital

Keywords:

Residual material, Cytocentrifugation, Utility, Cytospin preparation

Abstract

Background: Fine needle aspiration is currently the most favored technique for pre-operative diagnosis of most palpable and certain non-palpable masses. The present study aimed at exploring the utility of cytospin preparation from residual material in the needle hub for assisting in routine cytologic diagnosis.

Methods: For this prospective study, 100 cases of fine needle aspiration from lymph node, breast, soft tissue, thyroid and salivary glands were included. After preparation of routine smears, material in needle hub was rinsed in saline and cytospin preparation was made. Routine and cytospin preparations were assessed for cytologic diagnosis and results compared.

Results: Of the 100 cases included, the cytospin preparation showed good staining in all (100%). Cellularity was adequate in 90% of the cases with satisfactory cellular preservation in 72% cases. In 16 cases (16%), the diagnostic material was present in cytospin preparation while routine smears were inadequate for opinion.

 Conclusion: Cyto-centrifugation of the residual material in needle hub after fine needle aspiration improves the diagnostic yield of routine smears. Hence, this technique can be utilized to reduce the number of re-aspirations, especially at centers where the requisite equipment is already available.

Author Biographies

Shilpa Gupta, Hindu Rao Hospital

Department of Pathology

Lopamudra Deka, Hindu Rao Hospital

Department of Pathology

Ruchika Gupta, Chacha Nehru Bal Chikitsalaya

Department of Pathology

Kusum Gupta, Hindu Rao Hospital

Department of Pathology

Charanjeet Kaur, Hindu Rao Hospital

Department of Pathology

References

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Published

28-01-2015

How to Cite

1.
Gupta S, Deka L, Gupta R, Gupta K, Kaur C, Singh S. Cytospin preparation from residual material in needle hub: Does it add to fine needle aspiration diagnosis?. Ann of Pathol and Lab Med [Internet]. 2015 Jan. 28 [cited 2024 Dec. 23];2(1):A1-A5. Available from: https://pacificejournals.com/journal/index.php/apalm/article/view/198

Issue

Vol. 2 No. 1 (2015)

Section

Original Article

License

Copyright (c) 2015 Shilpa Gupta, Lopamudra Deka, Ruchika Gupta, Kusum Gupta, Charanjeet Kaur, Sompal Singh

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