Correlation of Interleukin-21 Levels With Clinical and Laboratory Parameters in ART Recipients

  • Manoj Kumar Meena Department of Microbiology, UCMS & GTB Hospital, Delhi
DOI: https://doi.org/10.21276/apalm.3250
Keywords: IL-21, Correlation, ART recipient, CD-4 count, CRP, Laboratory parameters

Abstract

Background: Interleukin-21 (IL-21) is a relatively new immune-enhancing, multifunctional and pleiotropic cytokine that plays an essential role in controlling chronic viral infections. It is a protein that in humans is encoded by IL-21 gene, mapped on chromosome 4 and 180 kb from IL-2 gene and the mRNA encoding the product is 616 nucleotides long. IL-21 is expressed in activated human CD4+ T cells but not in most other tissues. In addition, IL-21 expression is up-regulated by Th2 and Th17 subsets of T helper cells, as well as T follicular cells. Further IL-21 is expressed in NKT cells regulating the functions of these cells. IL-21 may be a critical factor in the control of persistent viral infections such as HIV. Present study aims to explore the levels of IL-21 in a select segment of ART recipients of East Delhi covered by NACP, Govt. of India. Methods: Estimation of levels of IL-21 was done using ELISA test employing commercially available kit (QAYEE-BIO Kit for Interleukin-21). The method of ELISA used for the present study was sandwich ELISA. Results: The mean levels of IL-21 in the study of 40 ART recipients were 1178.21±927.063. There were no controls included in the study. The range of IL-21 were 150-6100. In one patient IL-21 levels was not detected. Conclusion: This study attempts a clinical correlation with the levels of a cytokine with clinical and laboratory parameters which could help in a better understanding of the immunobiology of the disease. In the current study, the levels of IL-21 in samples of ART recipients were detected. A significant positive clinical correlation was seen between the levels of IL-21 and CD4 cell counts. IL-21 levels were high in those patients whose CD4 cell count was raised. There was a positive correlation between CD4 cell count and CRP. Therefore, further studies with a higher sample size may be required to arrive at a statistically significant correlation

References

Iannello A, Boulassel MR, Samarani S, Tremblay C, Toma E, Routy JP, et al. IL-21 enhances NK cell functions and survival in healthy and HIV-infected patients with minimal stimulation of viral replication. J Leukoc Biol 2010;87(5):857-67.

Kapil A, Bhaskaran CS. Human immunodeficiency virus: AIDS. In: Kapil A, Bhaskaran CS, editors. Textbook of Microbiology, 9th ed. Hyderabad: Universities Press (India) Pvt Ltd.; 2013:570-588.

Iannello A, Boulassel MR, Samarani S, Debbeche O, Tremblay C, Toma E, Routy JP, Ahmad A. Dynamics and consequences of IL-21 production in HIV-infected individuals: a longitudinal and cross-sectional study. J Immunol 2010;184(1):114-26.

Pallikkuth S, Parmigiani A, Pahwa S. Role of IL-21 and IL-21 receptor on B cells in HIV infection. Crit Rev Immunol 2012;32(2):173-95.

Gallo RC, Salahuddin SZ, Popovic M et al. Frequent detection and isolation of cytopathic retroviruses (HTLV III) from patients with AIDS and at risk for AIDS. Science 1984; 224: 500-3.

Tabib A, Greenland T, Mercier I, et al. Coronary lesions in young HIV-positive subjects at necropsy. Lancet 1992; 340:730.

Echevarria KL, Hardin TC, Smith JA. Hyperlipidemia associated with protease inhibitor therapy. Ann Pharmacother 1999; 33: 859-63.

Mirete G, Masia M, Gutierrez F, et al. Acute pancreatitis as a complication of ritonavir therapy in a patient with AIDS. Eur J Clin Microbiol Infect Dis 1998; 17: 810-11.

Hellerstein MK, Grunfeld C, Wu K, et al. Increased de novo hepatic lipogenesis in human immunodeficiency virus infection. J Clin Endocrinol Metab 1993; 76: 559-65.

Selik RM, Starcher ET, Curan JW. Opportunistic diseases reported in AIDS patients: frequencies, associations, and trends, and trends. AIDS 1987; 1: 175-182.

Sax PE. HIV Infection. In Cunha BA. Edited by Antibiotic E. New York: Physicians; 2005:227–33

Published
02-08-2023
Issue
Vol 10 No 4 (2023)
Section
Original Article

Copyright (c) 2023 Manoj Kumar Meena

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