Epidemiological study on human, cattle and rodent leptospirosis in South Gujarat region of India

Authors

  • Tanvi Harivadanbhai Panwala Microbiology department, Government Medical College, Surat, Gujarat.

Keywords:

eptospirosis, Microscopic Agglutination Test, PCR, Polymerize Chain Reaction

Abstract

Background: Surat district and neighbouring districts like Valsad, Navsari in South Gujarat region are considered as an endemic area for leptosprirosis. Types of leptospira serovars are depend on geographical area so it was important to find serovars which are predominant in humans, cattle and environment (soil, water) of this area.So aim of the study was to establish the transmission cycle of leprospirosis in this area by Isolation techniques and serology.

Methods: 207 human, 92 rodent and 258 animal blood samples were subjected to PCR, MAT test and culture in EMJH media. In addition, 550 animal urine, 7 rodent urine, 19 animal kidney and 571 rodent kidney were also tested by PCR.

Results: Out of total 207 suspected cases, 161 samples were positive by MAT and 94(45%) positive by Real time PCR. 51(24%) samples were positive by MAT and PCR both tests. In cattle and rodent, 65 and 9 serum samples were positive by MAT test for leptospirosis.Predominant serovars observed in human cases, cattle and rodents were L. Autumnalis, L.Canicola, L. Pomona and L. Icterohaemorrahgiae.

Conclusion:The current study reveals the presence of common leptospiral serovars, Autumnalis and Pomona infecting man, animals, and rodents, by serology and isolation. Hence this study reiterates the need for a strong and highly detailed control program for leptospirosis in this region, which should begin from the rodents and encompass the domestic animals as well.

 

DOI: 10.21276/APALM.1282

 

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Author Biography

Tanvi Harivadanbhai Panwala, Microbiology department, Government Medical College, Surat, Gujarat.

Microbiology department, Government Medical College,Surat.

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Published

25-10-2017

How to Cite

1.
Panwala TH. Epidemiological study on human, cattle and rodent leptospirosis in South Gujarat region of India. Ann of Pathol and Lab Med [Internet]. 2017 Oct. 25 [cited 2024 Nov. 19];4(5):A476-481. Available from: https://pacificejournals.com/journal/index.php/apalm/article/view/apalm1282

Issue

Vol. 4 No. 5 (2017)

Section

Original Article

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Copyright (c) 2017 Tanvi Harivadanbhai Panwala

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