Punica Granatum v/s Lawsonia Inermis: An in Vitro Anti-Fungal Study.

Authors

  • Pallav Singhal sarjug dental college and hostpital, affiliated to L.N. Mithila University, Darbhanga.
  • Narendra Nath Singh Kothiwal Dental College and research centre
  • Gadiputi Sreedhar Babu Banarasi Das college of dental sciences
  • Manu Batra Surendera Dental College and Research Institute
  • Sumita Bannerji The Dental College, Regional Institute of Medical Sciences, Imphal.
  • Soumi Ghanta haldia institute of dental sciences and research

Keywords:

Henna Leaves, Pomegranate peel, antifungal activity, candida.

Abstract

Background: Incidence of Fungal diseases is increasing with emerging immunodeficiency conditions. Discovery of antibiotics to combat these infections marked a resolution, but their inappropriate use blocked the action of antibiotics, change their target or ability to penetrate cells. Therefore there is a need for alternative potent antifungal products.

Aims & Objectives: To determine and compare the fungicidal action of pomegranate peel and henna leaves extract on candida.

Material & Methods: Herbal extract was prepared from sundried, powdered Pomegranate peel and henna leaves and stored at 4Ëšc until use. Candida was grown in Sabouraud Agar media by the sample collected from throat swab. Sterile filter disc immersed in extract was loaded onto the prepared plates with the control as clotrimazole disc. The zone of inhibition was measured at time interval of 18-24 hours after incubation and readings were statistically analysed.

Results: The Henna lemon extract was superior to the Pomegranate ethanol extract. The minimum inhibition zone for Pomegranate ethanol extract was 14.3±0.58 mm which is lesser than that of lawsonia lemon extract and standard antibiotic with 18.6±0.58 mm of inhibition zone.

Conclusion: The results showed that punica granatum and lawsonia inermis has a potent antifungal activity and the potential use of these products as cheap, nontoxic with less side-effects and as a convenient adjuvants to pharmaceutical antifungal products and need for further investigations to be used for clinical implications in humans.

Author Biographies

Pallav Singhal, sarjug dental college and hostpital, affiliated to L.N. Mithila University, Darbhanga.

MDS, Department of oral pathology and microbiology

as "Senior lecturer".

Narendra Nath Singh, Kothiwal Dental College and research centre

MDS,FICD, FPFA, Head of Department and P. G Guide, Dept. of Oral Pathology and Microbiology

Gadiputi Sreedhar, Babu Banarasi Das college of dental sciences

MDS, professor & head, Dept. of oral pathology and microbiology

Manu Batra, Surendera Dental College and Research Institute

MDS, Senior Lecturer, Department Of Public Health Dentistry

Sumita Bannerji, The Dental College, Regional Institute of Medical Sciences, Imphal.

MDS,Senior Lecturer, Dept. of Oral Pathology and Microbiology

Soumi Ghanta, haldia institute of dental sciences and research

MDS, Oral medicine and radiology, senior lecturer

References

1) Goldway M, Teff D, Schmidt R, Oppenheim AB, Koltin Y. Multidrug resistance in Candida albicans: disruption of the BENr gene. Antimicrob Agents Chemother1995;39(2):422-6.
2) White TC. Antifungal drug resistance in Candida albicans. ASM News 1997;63:427–433.
3) Saadabi MAA. “Evaluation of Lawsonia inermis L. (Sudanese Henna) Leaf extracts as an antimicrobial agent”. Res. J. Biol. Sci. 2007;2(4):419-423.
4) Dahham SS, Ali MR, Tabassum H, Khan M. Studies on Antibacterial and Antifungal Activity of Pomegranate (PunicagranatumL.). American-Eurasian J. Agric. & Environ. Sci.2010;9(3):273-281.
5) Orak HH, Demirci AS, Gumus T. Antifungal and Antibacterial Activity of Pomegranate (Punica GranatumL.CV.) Peel. EJEAFChe 2011;10(3):1958-69.
6) Ellepola ANB, Samaranayake LP. Oral candida infections and actinomycotics. Crit Rev Oral Biol Med 2000;11(2):172-98.
7) Ng KP, Kuan CS, Kaur H, Na SL, Atiya N, Velayuthan RD. Candida species epidemiology 2000–2013: a laboratory based report.Trop Med Int Health 2015. doi: 10.1111/tmi.12577
8) Casqueiro J, Alves C.Infections in patients with diabetes mellitus: A review of pathogenesis. Indian J EndocrinolMetab 2012;16(Suppl1):S27–S36.
9) Raju SB, Rajappa S. Isolation and Identification of Candida from the Oral Cavity. ISRN Dent 2011;2011:487921.
10) Kligman AM, Mescon H. The periodic-acid-schiff stain for the demonstration of fungi in animal tissue. J Bacteriol 1950;60(4):415–421.
11) RindumJL, Stenderup A, Holmstrup P. Identification of Candida albicanstypes related to healthy and pathological oral mucosa. J Oral Pathol Med 1994;23(9):406-12.
12) Xia H, Keane CT, Beattie S, O'Morain CA. Standardization of disk diffusion test and its clinical significance for susceptibility testing of metronidazole against Helicobacter pylori. Antimicrob Agents Chemother 1994;38(10):2357–2361.
13) Pai MB, Prashant GM, Murlikrishna KS, Shivakumar KM, Chandu GN. Antifungal efficacy of Punicagranatum, Acacia nilotica, Cuminumcyminum and Foeniculumvulgare on Candida albicans: An invitro study. Indian J Dent Res2010;21(3):334-6.
14) Endo EH Cortez DA, Ueda-Nakamura T, Nakamura CV, Dias Filho BP.Potent antifungal activity of extracts and pure compound isolated frompomegranate peels and synergism with fluconazole against Candida albicans. Res Microbiol 2010;161(7):534-540
15) Vasconcelos LC, Sampaio FC, Sampaio MC, Pereira Mdo S, HiginoJS, Peixoto MH.Inhibitory Concentration of Adherence of Punicagranatum Linn (pomegranate) Gel against S. mutans, S. Mitis and C. albicans. Braz Dent J 2006;17(3):223-7.
16) Miguel MG, Neves MA and Antunes MD. Pomegranate (PunicagranatumL.): A medicinal plantwith myriad biological properties - A short review. J. Med. Plants Res.2010;4(25):2836-2847.
17) KhanZS and Nasreen S.Phytochemical analysis, antifungal activity and mode of actionof methanol extracts from plants against pathogens. Journal of Agricultural Technology 2010;6(4):793-805
18) Lopez LI, Nery Flores SD, Silva BelmaresSY, Saenz Galindo A. Naphthoquinones: biological properties and synthesis of lawsone and derivatives - a structured review. Vitae 2014;21(3):248-258.
19) Babu PD, Subhasree RS. Antimicrobial Activities of Lawsonia inermis- A Review. Acad. J. Plant Sci. 2009;2(4):231-232.

Downloads

Published

17-08-2016

How to Cite

1.
Singhal P, Singh NN, Sreedhar G, Batra M, Bannerji S, Ghanta S. Punica Granatum v/s Lawsonia Inermis: An in Vitro Anti-Fungal Study. Ann of Pathol and Lab Med [Internet]. 2016 Aug. 17 [cited 2024 Nov. 19];3(3):A201-205. Available from: https://pacificejournals.com/journal/index.php/apalm/article/view/apalm592

Issue

Vol. 3 No. 3 (2016)

Section

Original Article

License

Copyright (c) 2016 Pallav Singhal, Narendra Nath Singh, Gadiputi Sreedhar, Manu Batra, Sumita Bannerji, Soumi Ghanta

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access at http://opcit.eprints.org/oacitation-biblio.html).