Prevalence of carbapenemases with detection of NDM-1 gene in nonfermenters isolated from a tertiary care hospital of north India

Authors

  • Mohan Lal Gupta BHU Varanasi
  • Nidhi Negi Sri Guru Ram Rai Institute of Medical & Health Sciences Patel Nagar Dehradun.
  • Pradyot Prakash BHU Varanasi
  • Malay Ranjan Sen BHU Varanasi

Keywords:

Carbapenemases, Metallo-β-lactamases (MBL), Minimal inhibitory concentration (MIC).

Abstract

Background: Increasing multidrug resistance in nonfermenters is of great concern to mankind because the drugs left in pipeline are only a few for the serious infections in hospital  ICU settings. The present study was performed to determine the prevalence of carbapenem resistance among nonfermenters and to characterize the most prevalent MBL genes (NDM-1)  by  polymerase chain reaction, among phenotypically MBL positive isolates in a tertiary care centre of north India.

Methods: A total of 383 clinical isolates comprising of 181 A. baumanii  and 182 P. aeruginosa  were included in this study. The A. baumanii  and  P. aeruginosa  were identified by using standard bacteriological techniques. All these isolates were screened for imipenem and meropenem resistance by Kirby-Bauer disk diffusion method and Minimal Inhibitory Concentration (MIC) methods according to CLSI guidelines.

Results: In the present study, the two nonfermenters (A. baumanii and P. aeruginosa) showed highest susceptibility to polymyxin-B (78.2%) followed by meropenem (70.5%), imipenem (66.4%), tigecyclin (57.6%) and combination of piperacillin-tazobactam (51.5%) by using disc diffusion method.

Conclusion: Treatment of infections caused by nonfermerters like P. aeruginosa and A. baumanii producing different carbampenemases including NDM-1 are now posing  serious challenge as these infections are resistant to almost all available antimicrobial agents.

Author Biographies

Mohan Lal Gupta, BHU Varanasi

MICROBIOLOGY

Nidhi Negi, Sri Guru Ram Rai Institute of Medical & Health Sciences Patel Nagar Dehradun.

Microbiology

Pradyot Prakash, BHU Varanasi

Microbiology

Malay Ranjan Sen, BHU Varanasi

Microbiology

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Published

06-11-2016

How to Cite

1.
Gupta ML, Negi N, Prakash P, Sen MR. Prevalence of carbapenemases with detection of NDM-1 gene in nonfermenters isolated from a tertiary care hospital of north India. Ann of Pathol and Lab Med [Internet]. 2016 Nov. 6 [cited 2024 Nov. 19];3(5):A368-373. Available from: https://pacificejournals.com/journal/index.php/apalm/article/view/apalm731

Issue

Vol. 3 No. 5 (2016): (Suppl) November

Section

Original Article

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Copyright (c) 2016 Mohan Lal Gupta, Nidhi Negi, Pradyot Prakash, Malay Ranjan Sen

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