Seroprevalence of Transfusion transmitted infections by using 4th generation Enzyme- linked immunosorbent assay kit: A 3 year study in a tertiary health care centre of Delhi

Authors

  • Mohammad Jaseem Hassan Hamdard Institute of Medical Sciences & Research (HIMSR), Hamdard University, New Delhi
  • Sabina Khan Department of Pathology, HIMSR, Hamdard University, New Delhi.
  • Zeeba Shamim Jairajpuri Department of Pathology, HIMSR, Hamdard University, New Delhi.
  • Safia Rana Department of Pathology, HIMSR, Hamdard University, New Delhi.
  • Salamah Parveen Imteyaz Department of Physiology, SMS&R, Sharda University, Greater Noida, U.P
  • Sujata Jetley Department of Pathology, HIMSR, Hamdard University, New Delhi.

Keywords:

Transfusion transmitted infections, Blood donors. HIV, Hepatitis B, Hepatitis C, Syphilis, Malaria

Abstract

Background: One of the greatest challenges of the blood transfusion services is to prevent the transmission of Transfusion transmitted infections (TTI), because these unsafe blood transfusions leads to increase morbidity and mortality and eventually leads to economic burden on the society. The objective of our study was to find out the seroprevalence of TTI among blood donors at our blood bank by using 4th generation ELISA kit and to compare our study with other studies conducted at national and state level.

Method: 2401 units of blood collected during 3 years period were screened for 5 infections. HIV, Hepatitis B and Hepatitis C infections were screened by using 4th generation ELISA kit. Test for syphilis was done by Rapid plasma reagin card test and test for malaria parasite was done by Advantage Mal card test.

Results: A total of 2401 units were collected during the period of three years. 98.25% donors were male. 91.09% donors were replacement donors. A total of 71 blood donors (2.95%) were tested positive for any one of the TTI. Out of these 71 cases, 69 were males and only 2 were females. The overall prevalence of HIV, HBV, HCV and syphilis in our study were 0.33%, 1.7%, 0.74% and 0.16% respectively. None of our donors was tested positive for malaria.

Conclusion: In order to minimize the risk of TTI, voluntary donors should be encouraged by means of educating general people about benefits of blood donation and motivating them by conducting regular blood camps.

References

1. Arora D, Arora B, Khetarpal A. seroprevalence of HIV, HBV, HCV and syphilis in blood donors in southern Haryana. Indian J Pathol Microbiol 2010;53:308-309.
2. Fernandes H, D’souza PF, D’souzaPM. Prevalence of transfusion transmitted infection in voluntary and replacement donors. Indian J Hamatol Blood Transfus 2010;26:89-91.
3. Raut MM, Joge US, Choudhary SG, Malkar VR, Ughade HM. Seroprevalence of transfusion transmitted infections among healthy blood donors at blood bank attached to a tertiary care hospital in Maharashtra state of India. International Journal of Health Sciences & Research 2012;2:18-24.
4. Contreras M (ed). ABC of transfusion (3rd edn). London BMJ Books, 1998.
5. Screening donated blood for transfusion-transmissible infections: recommendations. 2009, Geneva, World Health Organization.
6. National AIDS control organization. Standards for blood banks and blood transfusion services. New Delhi: Ministry of Health and family welfare Government of India; 2007
7. Widmann FK, editor. Technical manual American association of blood banks. Aglington USA. 1985:325-344.
8. Patel PA, Patel SP, Oza HV. Seroprevalence of transfusion transmitted infections (TTIs) in blood donors at western Ahmedabad- A secondary care hospital based study. Int J Biol Med Res. 2012;3:1806-1810.
9. “Manual on quality standards for HIV testing laboratories” produced and published by national AIDS control organization, Ministry of health and family welfare, Government of India, New Delhi published in 2007.
10. Global report: UNAIDS report on the global AIDS epidemic. 2010:6. http://www.unaids.org/globalreport/global_report.htm.
11. Pallavi P, Ganesh CK, Jayashree K, Manjunath GV. Seroprevalence and trends in transfusion transmitted infections among blood donors in a university hospital blood bank: a five year study. Indian J Hematol Blood 2011;27:1-6.
12. Sastry JM, agarwane SU, harke VA. Retrospective study of the five-year prevalence and trends of transfusion transmitted infections (TTIs) among blood donors at a charitable hospital blood bank in Pune, India. International J. of Healthcare and Biomedical Research 2014;2:193-200.
13. Kulkarni N. Analysis of the seroprevalence of HIV, HBsAg, HCV and syphilitic infections detected in the pretransfusion blood: A short report. International Journal of Blood Transfusion and Immunohematology 2012;2:1-3.
14. Pahuja S, Sharma M, Baitha B, Jain M. Prevalence and trends of markers of Hepatitis C virus, Hepatitis B virus, Human Immunodeficiency virus in Delhi blood donors: a hospital based study. Jpn J Infect. Dis. 2007;60:389-391.
15. Kakkar N, Kaur R, Dhanoa J. Voluntary donors- need for a second look. Indian J Pathol Microbiol 2004;47:381-383
16. Singh B, Verma M, Kotru M, Verma K and Batra M. Prevalence of HIV & VDRL seropositivity in blood donors of Delhi. Indian J Med Res 2005;122:234-236.
17. Karmakar PR, Shrivastava P, Ray TG. Seroprevalence of transfusion transmissible infections among blood donors at the blood bank of a Medical College of Kolkata. Indian J Public Health 2014;58:61-64.
18. Gupta R, Singh B, Singh DK, Chugh M. Prevalence and trends of transfusion transmitted infections in a regional blood transfusion centre. Asian J Transfus Sci. 2011;5:177-178.
19. Singh B, Kataria SP, Gupta R. Infectious markers in blood donors of East Delhi: prevalence and trends. Indian J pathol Microbiol 2004;47:477-479.
20. Sinha SK, Roychoudhary S, Biswas K, Biswas P, Bandopadhya R. prevalence of HIV, Hepatitis B, Hepatitis C and syphilis in donor’s blood: A study from eastern part of India. Open Journal of Hematology 2012;3
21. Kaur G, Basu S, Kaur R, Kaur P, Garg S. patterns of infections among blood donors in a tertiary care centre: A retrospective study. The National Medical Journal of India 2010;23(3): 147-149.
22. Adhikari L, Bhatta D, Tsering DC, Sharma DK, Pal R, Gupta A. Infectious disease markers in blood donors at central referral hospital, Gangtok, Sikkim. Asian J Transfus Sci 2010;4:412.
23. Ahmed Z, Umaru N, Shreesha K. Seroprevalence of transfusion transmitted infections among blood donors in Mangalore. Medica Innovatica 2012;1:24-26.
24. Chandra T, Kumar A, Gupta A. Prevalence of transfusion transmitted infections in blood donors: an Indian experience. Trop Doct 2009;39:152-154.
25. Khageshan AP, Kulkarni KR, baragundi MC. Seroreactivity of syphilis among blood donors of a blood bank. Annals of pathology and laboratory medicine 2016;3:A41-44

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Published

26-10-2016

How to Cite

1.
Hassan MJ, Khan S, Jairajpuri ZS, Rana S, Imteyaz SP, Jetley S. Seroprevalence of Transfusion transmitted infections by using 4th generation Enzyme- linked immunosorbent assay kit: A 3 year study in a tertiary health care centre of Delhi. Ann of Pathol and Lab Med [Internet]. 2016 Oct. 26 [cited 2024 Oct. 30];3(4):A333-339. Available from: https://pacificejournals.com/journal/index.php/apalm/article/view/apalm847

Issue

Vol. 3 No. 4 (2016)

Section

Original Article

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Copyright (c) 2016 Mohammad Jaseem Hassan, Sabina Khan, Zeeba Shamim Jairajpuri, Safia Rana, Salamah Parveen Imteyaz, Sujata Jetley

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