RBC Histogram as supplementary diagnostic tool with peripheral smear examination in evaluating anemias

Authors

  • Byna Syam Sundar Rao Ntr university of health sciences,Narayana medical college,Dept of pathology,Nellore.
  • Vissa Santhi
  • Nandam Mohan Rao
  • Bhavana Grandhi
  • Vijaya Lakshmi Murra Reddy
  • Praveena Siresala

Keywords:

RBC Histogram, Anaemia, Blood indices, Red cell distribution width.

Abstract

Background:  Red blood cell(RBC) histogram provide an idea about morphological changes of  red blood cells in hematological disorders. Peripheral smear examination findings are usually correlated with complete blood cell counts by automated analyzer. To known the utility and advantage of red cell histogram and correlation of microscopic examination of peripheral smear with automated histogram pattern.

 Methods: Blood  sample was collected from 220 anemia patients in  ethylene diamine tetra acetic acid (EDTA) tubes for peripheral smear examination and ran in Beckman coulter LH 780  automated hematology analyzer for obtaining histogram, complete blood count includes  hemoglobin, total leucocyte count, Platelet count, red blood cell indices and red cell distribution width. This  study was undertaken  for a period of one month  of  November  2016 in department of pathology,  central laboratory, narayana  medical college & hospital, Nellore.

 Results:  This study of histograms of  220 different types of  anemia consisted predominantly  females 154 (70%) , and  males 64(30%) . Maximum cases of anemia  were noted in 30-40 years of age range. Microcytic hypo chromic anemia was the most common (63.63%) followed by  normocytic normochromic anemia (19.4%), macrocytic  hypochromic anemia (2.2%), dimorphic anemia (12.72%) and pancytopenia (1.8%).

Left shifted curve and broad base mostly seen in microcytic anemia, right shift curve seen in macrocytic  anemia  and bimodal peak mostly seen in dimorphic anemia.

Conclusion: Histogram  can be used as  an important  screening  test for hematology and  can  become a new parameter  in the diagnosis of anemia though peripheral examination remains the definitive diagnostic  test for evaluation.

DOI: 10.21276/APALM.1468

Author Biography

Byna Syam Sundar Rao, Ntr university of health sciences,Narayana medical college,Dept of pathology,Nellore.

Dept of pathology,Narayana medical college, Nellore

References

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Published

19-12-2017

How to Cite

1.
Rao BSS, Santhi V, Rao NM, Grandhi B, Reddy VLM, Siresala P. RBC Histogram as supplementary diagnostic tool with peripheral smear examination in evaluating anemias. Ann of Pathol and Lab Med [Internet]. 2017 Dec. 19 [cited 2024 Dec. 26];4(6):A668-672. Available from: https://pacificejournals.com/journal/index.php/apalm/article/view/apalm1468

Issue

Vol. 4 No. 6 (2017)

Section

Original Article

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Copyright (c) 2017 Byna Syam Sundar Rao, Vissa Santhi, Nandam Mohan Rao, Bhavana Grandhi, Vijaya Lakshmi Murra Reddy, Praveena Siresala

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