Diagnostic Utility of Cell Block and Conventional Cytology in Evaluation of Lymph Node Aspirates

Sumiti Gupta; Ritu; Pooja Rathee; Davinder Kumar; Renuka Verma; Sunita Singh

doi:10.21276/apalm.3502

Authors

Sumiti Gupta Department of Pathology, Pandit Bhagwat Dayal Sharma Post Graduate Institute of Medical Sciences, Rohtak, Haryana, India
Ritu Department of Pathology, Pandit Bhagwat Dayal Sharma Post Graduate Institute of Medical Sciences, Rohtak, Haryana, India
Pooja Rathee Department of Pathology, Pandit Bhagwat Dayal Sharma Post Graduate Institute of Medical Sciences, Rohtak, Haryana, India
Davinder Kumar Department of General Surgery, University of Medical Sciences and GTB hospitals, New Delhi, India
Renuka Verma Department of Pathology, Pandit Bhagwat Dayal Sharma Post Graduate Institute of Medical Sciences, Rohtak, Haryana, India
Sunita Singh Department of Pathology, Pandit Bhagwat Dayal Sharma Post Graduate Institute of Medical Sciences, Rohtak, Haryana, India

DOI:

https://doi.org/10.21276/apalm.3502

Keywords:

Cell block, Cytosmear, Lymphadenopathy, FNAC, Histopathology

Abstract

Background: This study was conducted to assess the diagnostic utility of cell block and conventional cytology in the evaluation of lymph node aspirates.

Methods: This was a one-year prospective study approved by the ethical committee of the institution and was carried at a tertiary care hospital, Pt B.D. Sharma, PGIMS, Rohtak. This study included 46 patients attending the cytology outpatient department who were referred for fine needle aspiration from other clinical departments. FNAC was performed without anaesthesia, and the aspirated material was flushed on slides and then fixed immediately. The remaining materials were processed into cell blocks followed by H&E staining. The collected data was analysed using SPSS version 20 (IBM SPSS Statistics Inc., Chicago, Illinois, USA) Windows software program. A p-value <0.05 was taken as significant.

Results: The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of the combined cytosmear and cell block technique for cytopathological diagnosis were 100%, 75.8%, 78.59%, 100% and 85.39% respectively. The combined use of cytosmear and cell block led to increased diagnostic efficiency.

Conclusion: The combined use of FNAC smear and cell block can be useful for establishing a more definitive cytopathologic diagnosis of lymphadenopathy. Compared with FNAC, it gives a more accurate diagnostic architecture and apparent histopathological features.

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