Histopathological and Histomorphometric analysis of Pancreas and liver of diabetic rats treated with Mucuna Pruriens seed extract.
Keywords:
Histomorphometric analysis, Mucuna Pruriens, pancreas, Liver, DiabetesAbstract
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Background: Medicinal plants play a major role in controlling diabetes by producing microstructural changes in liver and pancreas. Quantification of islets and hepatocytes by using histomorphometric tools are effective in evaluating the antidiabetic action of drugs.
Aims and Objectives: To assess the anti diabetic properties of alcoholic extracts of Mucuna Pruriens seeds (200 mg/kg b.w) by means of histological and histomorphometric analysis of pancreas and liver .
Methods:Seventy two neonatal wistar rats were randomly divided into four groups, i.e. Normal , Diabetic control, diabetic rats treated with Mucuna and Glibenclamide. Diabetes was induced by a single intraperitoneal administration of streptozotocin (65mg/kg b.w) in 5 day old neonatal rats (n =54).12 weeks after the injection, animals were divided in to four groups with 18 animals in each. The study groups were as follows. Group A -Normal control, Group B – STZ untreated, Group C - Mucuna Pruriens 200mg/kg, Group D -Glibenclamide (1mg/kg).Fasting blood sugar levels were monitored once a week during the drug study .i.e. 12 weeks. At the end of every 4th week, 6 animals from each group were sacrificed for histological and histomorphometric studies on liver and Pancreas.
Results: From the histological studies, the diabetic group showed reduction of cell population  in  the pancreas and depletion of the number of hepatocytes in the liver. After 12 weeks of Drug treatment, These changes were markedly reduced in Mucuna Pruriens treated group. The obtained results were further quantified by means of Histomorphometric analysis and the results showed reduction in the number and diameter of islets, number of beta cells and number of healthy hepatocytes in diabetic control group and treatment with Mucuna Pruriens seed extract significantly reversed these changes (p < 0.05)
DOI:10.21276/APALM.1516
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