Establishing Reference Value of Biochemical Parameters: A Must Before Ensuring Quality in Biochemistry Diagnostic Lab

Authors

  • Gitanjali Goyal GGS Medical College, Faridkot, Punjab, India
  • K.M.D. Singh Panag GGS Medical College, Faridkot, Punjab

Keywords:

Reference value, Quality control, Diagnostic lab

Abstract

Background: Quality assurance in a laboratory comprises of a set of factors which besides numerous preanalytical, analytical and post analytical variables also include effect of age and sex on various parameters. Present study was done to know the impact of these factors on various biochemical variables and reference values of the various biochemical parameters were set according to age and sex.

Methodology: The present study was conducted on 200 apparently healthy individuals from Malwa belt of Punjab. The participants were divided according to age and sex. All the parameters were measured by standard lab protocols/methods.

Result: In addition to various preanalytical, analytical & post analytical variables, some of the parameters showed significant variations according to age and sex. Serum creatinine, uric acid, AST, ALP and Calcium showed significant difference in males as compared to females (p<0.05). Parameters like Urea, Creatinine, uric acid, total protein and cholesterol showed significant difference in individuals who were more than 50 years when compared with individuals less than 50 years (p<0.05).

Conclusion: These reference values will be used to compare the results of various biochemical parameters of patients coming to our tertiary care hospital. Also the setting of reference value should be done as a protocol before assuring quality control in the lab. But there is need for more elaborative studies and it should be comparable with the results from multiple laboratories that are using same methodology and instruments.

Author Biographies

Gitanjali Goyal, GGS Medical College, Faridkot, Punjab, India

Associate Professor, Deptt. of Biochemistry

K.M.D. Singh Panag, GGS Medical College, Faridkot, Punjab

Professor and Head, Deptt. of Biochemistry

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Published

17-08-2016

How to Cite

1.
Goyal G, Panag KS. Establishing Reference Value of Biochemical Parameters: A Must Before Ensuring Quality in Biochemistry Diagnostic Lab. Ann of Pathol and Lab Med [Internet]. 2016 Aug. 17 [cited 2024 Nov. 19];3(3):A212-216. Available from: https://pacificejournals.com/journal/index.php/apalm/article/view/apalm632

Issue

Vol. 3 No. 3 (2016)

Section

Original Article

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Copyright (c) 2016 Gitanjali Goyal, K.M.D. Singh Panag

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